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| 产品名称： | pRS414 |
|---|---|
| 商品货号： | TS193645 |
| Designations： | pRS414 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.788
Vector: pRS414 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescriptII KS+
Marker(s):TRP1,ampR
Construct size (kb): 4.788
Features: insert detection: lacZ marker(s): TRP1 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARSH4 replicon: f1 replicon: pMB1 MCS: KpnI...SacI centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuII--4.35, 0.45; BglI--3.1, 1.7; HindIII--3.85, 0.95. A YC-type shuttle vector with TRP1 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 |
| Related Products： | Component of: ATCC 87532 |
| Shipped： | frozen |
| Shipping Information： | Frozen phagemid in E. coli |