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| 产品名称： | pVE650 MA6618 |
|---|---|
| 商品货号： | TS193672 |
| Designations： | pVE650 MA6618 |
| Species： | Streptomyces avermitilis (ex Burg et al.) Kim and Goodfellow |
| Depositors： | Merck & Co., Inc. |
| Applications： | produces protein |
| Vector： | Construct size (kb): 47.79000091552734 |
| Insert： | DNA: genomic Insert lengths(kb): 24.0 Gene product: avrB |
| Insert Size (kb)： | 24.0 |
| Media： | ATCC® Medium 1339: R2 YE medium |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The plasmid contains the following BamHI fragments (kb): 24.05, 7.4, 4.7, 3.0, 2.35, 2.09, 1.85, 1.4, 1.09, 0.53, 0.02. Positioned between approximately 60-84 kb on the 110 kb map containing the avermectin gene cluster. avrC is included in a 4.28 kb BglII fragment and a 4.05 EcoRI fragment; and avrD is included in a 2.56 kb BglII fragment. The avrB, avrC, and avrD genes map to BglII fragments of 4.3, 0.1, and 2.6 kb at one end of the clone. |
| References： | MacNeil DJ, et al. Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector. Gene 111: 61-68, 1992. PubMed: 1547955 Gewain KM, et al. Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use. US Patent 5,252,474 dated Oct 12 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |