宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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pDRD2E5.6

货号 TS193684
中文名称 null
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产品名称: pDRD2E5.6
商品货号: TS193684
Designations: pDRD2E5.6
Species: Homo sapiens, human
Depositors: JR Kidd
Vector:
Construct size (kb): 8.5600004196167
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
marker(s): ampR
promoter: lac, T7, T3
replicon: pMB1, f1
Cross references:
Insert:
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DRD2
Genomic copy number: unique
Gene name: dopamine receptor D2
Contains complete coding sequence?: U
Chromosome: 11; Localization: 11 q22.2-q22.3
Type of DNA: genomic
Insert 5 end: EcoRI
Insert 3 end: EcoRI
Insert size (kb): 5.6
Cross references:
Insert lengths(kb): 5.599999904632568
Gene product: dopamine receptor D2 DRD2
Alleles: A1, B1, B2, A2
Insert Size (kb): 5.600
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 3.0; BamHI--8.0, 0.6; HindIII--8.6; PstI--4.0, 1.5, 1.35, 0.8, 0.5, 0.3; HindIII/PstI--3.4, 1.5, 1.3, 0.8, 0.8, 0.5, 0.3.
The insert contains a 4.5Kb TaqI fragment.
The downstream portion of pDRD2E5.6 overlaps the Suarez clone phD2-254.
References:

Hauge XY, et al. Detection and characterization of additional DNA polymorphisms in the dopamine D2 receptor gene. Genomics 10: 527-530, 1991. PubMed: 1679742

Suarez BK, et al. Linkage Disequilibria at the D2 dopamine receptor (DRD2) in alcoholics and controls. Genomics 19: 12-20, 1994. PubMed: 7910578

Judith R Kidd, personal communication