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| 产品名称： | pIB1 |
|---|---|
| 商品货号： | TS193692 |
| Designations： | pIB1 |
| Species： | Homo sapiens, human |
| Depositors： | Genentech, Inc., WH Dreger, Genentech, Inc. |
| Applications： | produces protein insulin |
| Vector： | Construct size (kb): 10.60000038146973 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: EcoRI Vector end: BamHI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: Synthetic DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: INS Genomic copy number: unique Gene name: insulin Contains complete coding sequence?: U Chromosome: 11; Localization: 11 p15.5 Type of DNA: synthetic Insert end: EcoRI Insert end: BamHI Insert size (kb): 0.1 Cross references: Insert lengths(kb): 0.1000000014901161 Gene product: insulin INS Alleles: K2, A3, B2, A1, A1, A4, A3, A3, A5, A6, B1, K1, A1, A2, A2, A2 Target Gene: insulin |
| Insert Size (kb)： | 0.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests on an agarose gel give the following sizes (kb): EcoRI--6.4, 4.1; BamHI--10.5. The sequence of the insert is AATTCATGTT CGTCAATCAG CACCTTTGTG GTTCTCACCT CGTTGAAGCT TTGTACCTTG TTTGCGGTGA ACGTGGTTTC TTCTACACTC CTAAGACTTA ATAGGATC, including a HindIII site at position 46. This construct directs the synthesis of a beta-galactosidase-insulin B chain fusion protein regulated by the lac promoter. The B peptide can be released by cyanogen bromide cleavage and used to reconstitute insulin with the product of pIA1, ATCC 31448. This clone was constructed from pBR322, an EcoRI fragment from lambda plac containing most of the beta-galactosidase gene, and synthetic DNA encoding human insulin B chain. |
| References： | Riggs AD. Method for microbial polypeptide expression. US Patent 4,366,246 dated Dec 28 1982 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |