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| 产品名称： | pRCgammaA-2 |
|---|---|
| 商品货号： | TS193775 |
| Designations： | pRCgammaA-2 |
| GenBank Number： | X52815 |
| Species： | Rattus norvegicus, rat |
| Depositors： | CW Brown |
| Vector： | Construct size (kb): 4.809999942779541 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.845999956130981 Tissue: kidney Gene product: actin, gamma 1 ACTG1 |
| Insert Size (kb)： | 1.846 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following unique sites in nucleotides from the 5 end: KpnI--931, XbaI--1148, HindIII--1283. Restriction digests of the clone give the following sizes (kb): BamHI--4.8; HindIII--3.6, 1.2; PstI--3.0, 1.8; EcoRI--4.8; XbaI/XhoI--3.0, 1.1, 0.7. At the 5 end, the insert includes poly(G)11 and 12 5 untranslated nucleotides. The insert contains a complete open reading frame (nucleotides 23 - 1148), and extends to the PstI site at 1848 just 5 to the poly(A). The 3 untranslated region contains 2 inverted repeat sequences and a consensus TATA transcription initiation signal. The insert is oriented such that transcription from the T7 promoter gives the sense strand. |
| References： | Brown CW, et al. A cDNA sequence encoding cytoskeletal gamma-actin from rat. Nucleic Acids Res. 18: 5312, 1990. PubMed: 2402472 Chester W Brown, personal communication |