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| 产品名称： | HCL-1 |
|---|---|
| 商品货号： | TS193862 |
| Designations： | HCL-1 |
| Species： | Homo sapiens, human |
| Depositors： | Y Israel |
| Vector： | Construct size (kb): 4.099999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II SK- Intact vector size: 2.961 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR replicon: pMB1, f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CTH Genomic copy number: unique Gene name: cystathionase (cystathionine gamma-lyase) Contains complete coding sequence?: U Chromosome: 16; Localization: 16 Tissue: liver Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.1 Cross references: Insert lengths(kb): 1.100000023841858 Tissue: liver Gene product: cystathionine gamma-lyase(cystathionase (cystathionine gamma-lyase)) CTH |
| Insert Size (kb)： | 1.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.1; BglII--4.1; BamHI--4.1; HindIII--4.1; XbaI--4.1. The insert contains the following restriction sites (bp from the 5 end): BglI--88; BamHI--114; AvaII--128; AvaI--198; ScaI--278; DraI--333; XmnI--589; BstEII--840; BspHI--959; NsiI--968; BglII--1065; XbaI--1068; HindIII--1076. Contains a complete coding sequence, as a variant missing 132 bp included in other cDNAs derived from liver. Detects a 2.5 kb mRNA in human liver. Transcription from the T3 promoter gives the sense strand. Enzymes not detecting polymorphism: TaqI, XbaI, BglI, RsaI, HindIII, MspI, PstI, EcoRV, ScaI, SacI, BamHI, NcoI, Sau3A, PvuII. |
| References： | Chua AO, et al. Expression cloning of a human IL-12 receptor component--a new member of the cytokine receptor superfamily with strong homology to gp130. J. Immunol. 153: 128-136, 1994. PubMed: 7911493 Lu Y, et al. Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase. Biochem. Biophys. Res. Commun. 189: 749-758, 1992. PubMed: 1339280 The last 13 nucleotides of the coding sequence are missing.---ATCC staff |