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微信小章| 产品名称： | hTERT RPE-1 |
|---|---|
| 商品货号： | TS193917 |
| Organism： | Homo sapiens, human |
| Tissue： | Retina, eye; pigmented epithelium |
| Cell Type： | Epithelial cells immortalized with hTERT |
| Product Format： | frozen |
| Morphology： | Epithelial-like |
| Culture Properties： | Adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Gender： | Female |
| Storage Conditions： | Liquid nitrogen vapor phase |
| Karyotype： | This is a near-diploid human cell line of female origin with a modal chromosome number of 46 that occurred in 90% of the cells counted. The sex chromosomes consist of a karyotypically normal X-chromosome and a derivative X-chromosome with additional chromosomal material at the terminal end of the q-arm. The derivative X-chromosome was present in all of the cells analyzed. |
| Images： |  |
| Derivation： | The hTERT-immortalized retinal pigment epithelial cell line, hTERT RPE-1, was derived by transfecting the RPE-340 cell line with the pGRN145 hTERT-expressing plasmid (ATCC MBA-141).xa0 Cells were cultured in medium containing hygromycin B until stable clones were selected Pubmed: 9454332, 9916802. |
| Antigen Expression： | The cells express the Ep-16 antigen as determined by flow cytometry using the Ep-16 monoclonal antibody (ATCC HB-155), and cytokeratins as determined by immunocytochemistry using a pan-cytokeratin antibody. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:5 to 1:10 twice weekly
Medium Renewal: Every 2 days (or as needed)
Note: Subculture when cell concentration reaches between 2 X 104 and 4 X 104 cells/cm2.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005 |
| Cryopreservation： | Complete growth medium supplemented with an additional 60% fetal bovine serum and 10% DMSO. Store in liquid nitrogen vapor. Avoid immersing vials into liquid nitrogen. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 12,14 D13S317: 11,12 D16S539: 11 D5S818: 11 D7S820: 10,11 THO1: 9 TPOX: 8 vWA: 17,18 |
| Population Doubling Level (PDL)： | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
| Name of Depositor： | Geron Corporation |
| Deposited As： | Homo sapiens |
| References： | Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Jiang XR, et al. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype. Nat. Genet. 21: 111-114, 1999. PubMed: 9916802 Matsunaga H, et al. Beta-galactosidase histochemistry and telomere loss in senescent retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 40: 197-202, 1999. PubMed: 9888444 Freshney RI. Culture of Animal Cells: A Manual of Basic Technique, 5th edition. New York: Wiley Liss; 2005. For more information on enzymatic dissociation and subculturing of cell lines see Chapter 13. Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Erfle H, et al. Reverse transfection on cell arrays for high content screening microscopy. Nat Protocxa02(2):392-9, 2007. PubMed: 17406600 Kabeche L, Compton DA. Cyclinxa0Axa0regulatesxa0kinetochorexa0microtubulesxa0toxa0promotexa0faithfulxa0chromosomexa0segregation. Naturexa0502(7469):110-3, 2013. PubMed: 24013174 Erfle H, et al. Workxa0flowxa0forxa0multiplexingxa0siRNAxa0assaysxa0byxa0solid-phasexa0reversexa0transfectionxa0inxa0multiwellxa0plates. J Biomol Screenxa013(7):575-80, 2008. PubMed: 18599879 Fink J, et al. Comparativexa0studyxa0andxa0improvementxa0ofxa0currentxa0cellxa0micro-patterningxa0techniques. Lab Chipxa07(6):672-80, 2007. PubMed: 17538708 |