微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pUC1813-NoriT |
|---|---|
| 商品货号： | TS193922 |
| Designations： | pUC1813-NoriT |
| Species： | pCU1 |
| Depositors： | PN Hengen |
| Vector： | Construct size (kb): 4.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 1.5 Gene product: (IncN) origin of transfer replication oriT |
| Insert Size (kb)： | 1.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.5; HindIII--2.6, 1.6; BamHI--2.7, 1.5; PstI--2.55, 1.65. Plasmid containing an oriT cassette bounded by multiple restriction sites for ease in subcloning. The order of elements in the polylinker is: HindIII PstI XbaI BamHI SmaI KpnI - insert EcoRI HindIII oriT BamHI EcoRI insert - SmaI BamHI XbaI PstI HindIII. |
| References： | Hengen PN, Iyer VN. DNA cassettes containing the origin of transer (oriT) of two broad-host-range transfer systems. BioTechniques 13: 56-62, 1992. PubMed: 1503776 |