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| 产品名称： | Rickettsia tamurai |
|---|---|
| 商品货号： | TS193959 |
| Classification： | Rickettsiaceae, Rickettsieae |
| Agent： | Rickettsia tamurai |
| Strain： | AT-1 |
| Positive for Mycoplasma： | Yes |
| Biosafety Level： | 3
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -80°C or colderxa0xa0 |
| Comments： | The AT-1 strain is recognized as the type strain for a newly identified rickettsial species (Rickettsia tamurae), a gram -negative, obligately intracellular and non-motile bacterium. The preparation available through ATCC tested positive for mycoplasma (Mycoplasma orale) contamination by PCR. Requests for this agent require special permits and a signed statement assuming all risks and responsibility for laboratory handling. |
| Effect on Host： | Bacteria visible in cells after staining with Gimenez stain. |
| Recommended Host： | Vero cells (ATCC CRL-1587) |
| Growth Conditions： | Recommendations for Infection: 5 days at 32°C, a 5% CO2 in air atmosphere is recommended in EMEM (ATCC 30-2003); with 2mM L-glutamine supplemented with 2% FBS (ATCC 30-2020), following centrifugation at 700 g for one hour at 22°C |
| Name of Depositor： | Dr. P. -E. Fournier |
| Source： | Isolated from Amblyomma testudinarium nymph in Anan, Tokushima prefecture, Japan, in 1993 |
| References： | Marrero, M and Raoult D. Centrifugation-shell vial technique for rapid detection of Mediterranean spotted fever rickettsia in blood culture. Am J Trop Med Hyg. 1989 Feb;40(2):197-9. PubMed: 2645804 Fournier, P. E. et. al. Rickettsia tamurae sp. nov., isolated from Amblyomma testudinarium ticks. Int J Syst Evol Microbiol. 2006 Jul;56(Pt 7):1673-75. PubMed: 16825648 |