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| 产品名称： | pKB132 |
|---|---|
| 商品货号： | TS193978 |
| Designations： | pKB132 |
| GenBank Number： | M73626 |
| Species： | Sonchus yellow net virus |
| Depositors： | AO Jackson |
| Vector： | Construct size (kb): 0.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.7009999752044678 Gene product: glycoprotein (viral envelope) G |
| Insert Size (kb)： | 0.701 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.2, 0.7; PvuII--2.8, 1.1; NdeI/HindIII--3.2, 0.7. The insert contains the following restriction sites (nt from the 5 end): NdeI--60; EcoRV--671, 678. Includes a portion of the coding sequence (corresponding to part of the carboxy terminal region). The complete coding region extends from nt 35-1933 of the published sequence. Subcloned from pAS3-9. The 5 end of the insert (which is the 5 portion of the carboxy portion of the ORF) is adjacent to the EcoRI site of the MCS. |
| Classification： | Nucleorhabdovirus |
| References： | Goldberg KB, et al. Structure of the glycoprotein gene of Sonchus yellow net virus, a plant rhabdovirus. Virology 185: 32-38, 1991. PubMed: 1926779 Andrew O Jackson, personal communication |