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| 产品名称： | pRS423 |
|---|---|
| 商品货号： | TS194074 |
| Designations： | pRS423 |
| Depositors： | TW Christianson |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.797 Vector: pRS423 (phagemid) Promoters: Promoter T7 (phi10) Construction: pJS92, pRS303, pBluescript II SK+ Marker(s):ampR,HIS3 Construct size (kb): 5.797 Features: insert detection: lacZ marker(s): HIS3 marker(s): ampR promoter: T3 promoter: T7 (phi10) promoter: lac replicon: 2 micron replicon: f1 replicon: pMB1 MCS: KpnI...SacI |
| Applications： | YE-type (episomal) shuttle vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; PvuI--3.7, 2.1. Contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.3 + or - 1.3% of progeny per doubling. YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. The beta-galactosidase alpha peptide permits blue-white detection of recombinants. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Christianson TW, et al. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992. PubMed: 1544568 |
| Related Products： | component of:ATCC 77108 component of:ATCC 87531 component of:ATCC 87535 |
| Shipped： | frozen |
| Shipping Information： | Freeze dried phagemid in E. coli |