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| 产品名称： | pH3a |
|---|---|
| 商品货号： | TS194120 |
| Designations： | pH3a |
| GenBank Number： | X58968 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 3.802999973297119 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: MMP2 Other gene symbols: CLG4A; CLG4 Gene name: matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase) Contains complete coding sequence?: U Chromosome: 16; Localization: 16 q12.2 Tissue: placenta Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.117 Cross references: DNA Seq. Acc.: X58968 Insert lengths(kb): 1.116999983787537 Tissue: placenta Gene product: matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)(collagenase IVA (basement membrane), metalloproteinase stromelysin-2) CLG4 |
| Insert Size (kb)： | 1.117 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.15; BamHI--3.85; HindIII--3.85; PvuII--2.35, 1.2, 0.3; PstI--3.85. The insert contains the following restriction sites (bp from the 5 end): AvaI--204, 253; HaeIII--75, 111, 180, 221, 252, 558, 757, 889, 988; MboI--497, 604, 616, 635, 745; PvuII--973. Contains 236 bp of the 5 untranslated region (starting 50 bp downstream of the transcription start point) and encodes the amino terminal portion of the protein. Detects a transcript of 3.1 kb. |
| References： | Levy AT, et al. Increased expression of the Mr 72,000 type IV collagenase in human colonic adenocarcinoma. Cancer Res. 51: 439-444, 1991. PubMed: 1846313 William Stetler-Stevenson, personal communication |