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| 产品名称： | p425 MET25 |
|---|---|
| 商品货号： | TS194182 |
| Designations： | p425 MET25 |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.461 Vector: p425 MET25 (plasmid) Promoters: Promoter for expression MET25 Construction: pRS425, MET25 promoter Marker(s):LEU2,ampR Construct size (kb): 7.461 Features: marker(s): LEU2 marker(s): ampR promoter for expression: MET25 replicon: 2 micron MCS: XhoI...SpeI terminator: CYC1 |
| Applications： | YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--4.0, 3.0, 0.4; EcoRI--5.2, 2.3; EcoRV--5.1, 2.4. Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter is repressed when cells are grown in the presence of methionine. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | frozen |