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微信小章| 产品名称： | Murine pneumonia virus |
|---|---|
| 商品货号： | TS194232 |
| Classification： | Paramyxoviridae, Pneumovirus |
| Deposited As： | Murine pneumonia virus |
| Agent： | Murine pneumonia virus |
| Strain： | 15 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | VR-1819™ was treated for Mycoplasma sp. at the ATCC® by three passages with media supplemented with an antibiotic (Mycoplasma Removal Agent – Bio-Rad catalog BUF035).xa0 The original item number was VR-25™. |
| Effect on Host： | CPE, rounding and degeneration |
| Recommended Host： | BHK-21 C-13 (ATCC® CCL-10™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 2-3 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation:xa05-12 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | J Parker |
| Source： | Lungs of mice, 1939 |
| References： | Horsfall F, Hahn RG. A pneumonia virus of Swiss mice. Proc. Soc. Exp. Biol. Med. 40: 684-686, 1939. Horsfall F, Hahn RG. A latent virus in normal mice capable of producing pneumonia in its natural host. J. Exp. Med. 71: 391-408, 1940. Tennant RW, Ward TG. Pneumonia virus of mice (PVM) in cell culture. Proc. Soc. Exp. Biol. Med. 111: 395-398, 1962. PubMed: 13980577 |