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| 产品名称： | VHL cosmid 11 in Escherichia coli |
|---|---|
| 商品货号： | TS194247 |
| Designations： | VHL cosmid 11 in Escherichia coli |
| GenBank Definition： | Homo sapiens von Hippel-Lindau tumor suppressor (VHL) gene, complete cds |
| GenBank Number： | AF010238 |
| Depositors： | I Kuzmin, National Cancer Institute |
| Vector： | Construct Information Total size of construct (kb): 48.5 Marker: ampR Vector Information Vector: pWek Vector type: cosmid Vector size (kb): 8.5 Markers: ampR |
| Insert： | Tissue: renal cell carcinoma Disease: von Hippel-Lindau tumor Type of DNA: genomic Insert ends: BamHI, Sau3AI Insert size (kb): 40.0 Genbank accession: AF010238 (partial sequence) |
| Insert Size (kb)： | 40.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Frozen glycerol stock of E. coli containing the cosmid. |
| Comments： | Contains exon 1 of VHL disease tumor suppressor gene critical region deleted in renal cell carcinomas and approx. 30kb region upstream. |
| References： | Latif F, et al. Identification of the von Hippel-Lindau disease Tumor Suppressor Gene. Science 260: 1317-1320, 1993. PubMed: 8493574 Kuzmin I, et al. One-megabase yeast artificial chromosome and 400-kilobase cosmid-phage contigs containing the von Hippel-Lindau tumor suppressor and Ca(2+)-transporting adenosine triphosphatase isoform 2 genes. Cancer Res. 54: 2486-2491, 1994. PubMed: 8162598 |