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| 产品名称： | pGH101 |
|---|---|
| 商品货号： | TS194261 |
| Designations： | pGH101 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | GE Herman |
| Vector： | Construct size (kb): 7.599999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 3.200000047683716 Gene product: beta-galactosidase(beta-D-galactosidase) lacZ |
| Insert Size (kb)： | 3.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The lacZ cassette can be excised with either BamHI or SalI. This plasmid is suitable for the study of eukaryotic expression. It contains a functional lacZ gene without any regulatory elements. The lacZ gene is a BamHI fragment from pMC1871 and is lacking 8 non-essential N-terminal amino acids. Linkers provide an inframe ATG and new unique BamHI and SalI sites on both ends of the lacZ fragment. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Herman GE, et al. An E. coli beta-galactosidase cassette suitable for study of eukaryotic expression. Nucleic Acids Res. 14: 7130, 1986. PubMed: 3093988 Casadaban MJ, et al. Beta-galactosidase gene fusions for analyzing gene expression in Escherichia coli and yeast. Methods Enzymol. 100: 293-308, 1983. PubMed: 6312261 |