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| 产品名称： | pJ5omega |
|---|---|
| 商品货号： | TS194318 |
| Designations： | pJ5omega |
| Depositors： | H Land |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.7000000476837160 Vector: pJ5omega (plasmid) Promoters: Promoter MMTV LTR Construction: pBR322, SV40, MMTV Marker(s):ampR Construct size (kb): 3.700000047683716 Features: marker(s): ampR promoter: MMTV LTR replicon: pMB1 terminator: SV40 |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.7; HindIII--3.7; BamHI--3.7; SmaI--3.7; PvuI--2.2, 1.5. Demonstrates expression inducible by dexamethasone. A mammalian shuttle vector for expression of a sequence cloned into the polylinker site under the control of the MMTV LTR promoter. Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), and MMTV LTR (-360 to +150, glucocorticoid regulatory sequences, cap site). |
| Media： | ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Morgenstern JP, Land H. A series of mammalian expression vectors and characterisation of their expression of a reporter gene in stably and transiently transfected cells. Nucleic Acids Res. 18: 1068, 1990. PubMed: 2156225 Hartmut Land, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |