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| 产品名称： | pspT19WDV1 |
|---|---|
| 商品货号： | TS194437 |
| Designations： | pspT19WDV1 |
| Species： | wheat dwarf geminivirus |
| Depositors： | PM Mullineaux |
| Vector： | Construct size (kb): 4.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 1.0900000333786 Gene product: ORF 1, 10 kDa |
| Insert Size (kb)： | 1.090 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI/BamHI--3.2, 1.1; BamHI--4.1. A NlaIII fragment extending from nt 213 to nt 1303. The NlaIII fragment was cloned into the SmaI site of pUC18. The SstI-(T4 polI)-BamHI fragment was isolated and inserted into pspT19. The plasmid can be linearized with SalI for SP6 transcription in vitro. |
| Classification： | Geminivirus |
| References： | Woolston CJ, et al. Agroinfection and nucleotide sequence of cloned wheat dwarf virus DNA. Plant Mol. Biol. 11: 35-43, 1988. P M Mullineaux, personal communication |