| 产品名称: | pEKZ104.1 |
|---|---|
| 商品货号: | TS194569 |
| Designations: | pEKZ104.1 |
| Species: | Homo sapiens, human |
| Depositors: | RL White, Y Nakamura |
| Applications: | Codominant segregation of the MspI RFLP was observed in 41 three-generation families. |
| Vector: | Construct size (kb): 6.599999904632568 |
| Insert: | DNA: genomic Insert lengths(kb): 4.0 Gene product: DNA Segment, single copy D15S30 Alleles: A1, A2 |
| Insert Size (kb): | 4.0 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--6.6; PstI--3.5, 0.99, 0.83, 0.58, 0.47; BamHI--6.6; EcoRI/HindIII--2.5, 2.0, 1.85; HindIII--4.2, 1.85; consistent with deposited DNA. An EcoRI/HindIII double digest shows insert fragment sizes of 2.0 and 1.8 kb. Codominant segregation of the MspI RFLP was observed in 41 three-generation families. Enzyme(s) not detecting polymorphism: BamHI, HindIII, PstI, PvuII, RsaI and TaqI. |
| References: | Kumlin-Wolff E, et al. Isolation and mapping of a polymorphic DNA sequence pEKZ104.1 on chromosome 15 D15S30. Nucleic Acids Res. 16: 1224, 1988. PubMed: 2449658 Yusuke Nakamura, personal communication |