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微信小章| 产品名称： | EWD299 |
|---|---|
| 商品货号： | TS194571 |
| Designations： | EWD299 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | WS Dallas |
| Applications： | detection of LT toxin produces protein heat-labile enterotoxin |
| Vector： | Construct size (kb): 10.10000038146973 |
| Insert： | DNA: genomic Insert lengths(kb): 1.799999952316284 Gene product: heat-labile enterotoxin lt Target Gene: LT toxin, heat-labile enterotoxin |
| Insert Size (kb)： | 1.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Freeze dried phagemid in E. coli |
| Comments： | The insert contains internal HincII, EcoRI and HindIII sites. The plasmid exists in DH1 as a multimer. Digestion of the plasmid with HindIII yields a 0.85 kb fragment containing nucleotide sequence for the entire B subunit and about one-third of the A subunit. This is a deletion mutant of EWD022 and seems to contain the minimum coding sequence to obtain functional proteins. This expresses proteins of 22.5 kDa and 11.5 kDa, having both LT and adenylate cyclase-activating activity. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Dallas WS, et al. Cistrons encoding Escherichia coli heat-labile toxin. J. Bacteriol. 139: 850-858, 1979. PubMed: 383697 U.S. Food & Drug Administration Enterotoxigenic Escherichia coli: Heat-stabile enterotoxin (human), heat-stabile enterotoxin (porcine), and heat-labile enterotoxin In: U.S. Food & Drug Administration Bacteriological analytical manual 8th ed. Gaithersburg, MD. AOAC International pp. 24.12-24.14, 1995 AOAC International Detection of Escherichia coli producing heat-labile enterotoxin. DNA colony hybridization method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 984.34. Guzman-Verduzco LM, Kupersztoch YM. Fusion of Escherichia coli heat-stable enterotoxin and heat-labile enterotoxin B subunit. J. Bacteriol. 169: 5201-5208, 1987. PubMed: 3312169 |