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| 产品名称： | Rat cytomegalovirus |
|---|---|
| 商品货号： | TS194631 |
| Classification： | Herpesviridae, Muromegalovirus |
| Agent： | Rat cytomegalovirus |
| Strain： | Priscott |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | tissue, animal Australia Isolation date: 1979 |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | High concentrations of virus in liver and spleen 14 days after i.c. inoculation. |
| Effect on Host： | Yes, in vitro effects: cytopathic effects (focal rounding) in tissue culture CPE, refractile rounding in TC; death of newborn rats, often with ataxia. Yes, in vivo effects: death of newborn rats, often with ataxia |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
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| Recommended Host： | Recommended Host:xa0 Rat2 (ATCC CRL-1764) |
| Growth Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 8-12 days; For best results cells should be 24 to 48 hours old and 70-90% confluent (not 100% confluent). |
| Name of Depositor： | PK Priscott |
| Source： | tissue, animal Australia Isolation date: 1979 |
| Year of Origin： | 1979 |
| References： | Priscott PK, Tyrrell DA. The isolation and partial characterisation of a cytomegalovirus from the brown rat, Rattus norvegicus. Arch. Virol. 73: 145-160, 1982. PubMed: 6293409 newborn wild brown rat Rattus norvegicus, sub-maxillary gland |