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| 产品名称： | pSV5-neo plasmid in E. coli |
|---|---|
| 商品货号： | TS194657 |
| Designations： | pSV5-neo plasmid in E. coli |
| Depositors： | P Berg |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.2 Vector: pSV5-neo plasmid in E. coli (plasmid) Promoters: Promoter SV40 early Construction: SV40, Tn5, pBR322, early region of polyoma virus Marker(s):G418R,ampR Construct size (kb): 9.2 Features: marker(s): ampR, G418R promoter: SV40 early replicon: pMB1 |
| Applications： | expression vector shuttle vector |
| Comments： | Provides dominant selectable marker for resistance to antibiotic G418 in mammalian cell lines. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |