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| 产品名称： | pAKA16 |
|---|---|
| 商品货号： | TS194668 |
| Designations： | pAKA16 |
| Depositors： | JG Coote, AK Azad |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.3000001907348630 Vector: pAKA16 (plasmid) Construction: pPH843, pIC20H Marker(s):ampR Construct size (kb): 5.300000190734863 Features: insert detection: lacZ marker(s): ampR other: mob replicon: ori(P) MCS: HindIII/SphI/PstI/.../NruI/HindIII |
| Applications： | shuttle vector vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.3; BamHI--5.3; PstI--3.5, 1.75. The unique Bsp1286I is located within the ori(P). Shuttle vector that can be mobilized by conjugation between E. coli and Pasteurella haemolytica. Constructed by ligating a 979 bp ApaLI fragment from pIC20H (which contains lacZalpha, MCS, and lacIPO) to ApaLI-digested pPH843. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Azad AK, et al. Construction of conjugative shuttle and suicide vectors for Pasteurella haemolytica and P. multocida. Gene 145: 81-85, 1994. PubMed: 8045428 Azad AK, et al. Distinct plasmid profiles of Pasteurella haemolytica serotypes and the characterizaton and amplification in Escherichia coli of ampicillin-resistance plasmids encoding ROB-1 beta-lactamase. J. Gen. Microbiol. 138: 1185-1196, 1992. PubMed: 1527493 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried E. coli containing the plasmid |