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| 产品名称： | KIM185 |
|---|---|
| 商品货号： | TS194693 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism PubMed: 7690325. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Animals were immunized with CR3 (CD11b/CD18). Spleen cells were fused with Sp2/0 myeloma cells. KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism PubMed: 7690325. The antibody recognizes an epitope distinct from the KIM127 (ATCC CRL-2838) antibody. |
| Comments： | Animals were immunized with CR3 (CD11b/CD18). Spleen cells were fused with Sp2/0 myeloma cells. KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism PubMed: 7690325. The antibody recognizes an epitope distinct from the KIM127 (ATCC CRL-2838) antibody. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Protocol: Shake off attached cells and scrape if necessary.
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(4) to 1 X 10(5) viable cells/ml. Interval: Maintain cultures at a cell concentration between 2.5 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium with 20% fetal bovine serum and 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | mouse IgG1 kappa |
| Population Doubling Time： | 20 hours |
| Name of Depositor： | MK Robinson |
| Year of Origin： | 1989 |
| References： | Andrew D, et al. KIM185, a monoclonal antibody to CD18 which induces a change in the conformation of CD18 and promotes both LFA-1- and CR3-dependent adhesion. Eur. J. Immunol. 23: 2217-2222, 1993. PubMed: 7690325 |