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产品简介
购买须知
| 产品名称： | pE25.a pBS300 |
|---|---|
| 商品货号： | TS194711 |
| Designations： | pE25.a pBS300 |
| Depositors： | IE Scheffler |
| Vector： | Vector: pE25.a Size: 3,200 xa0bpxa0 Selectable Marker:xa0 ampicillin resistant Cloning sites: EcoR I, Sac I, Kpn I, Sma I, BamHI,xa0Xba I, Sal I, Pst I, Sph I, Hind III Replicon: pUC (pMB1) Features:xa0 Lac, T3 and T7 promoters |
| Insert： | DNA: genomic
Insert lengths(kb): 0.349
Tissue: DNA from hamster/human XJM12.1.3 hybrid cell line
Gene product: DNA Segment, repetitive D1Z7 |
| Insert Size (kb)： | 0.349 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Plasmid in Escherichia coli |
| Comments： | The insert contains the following sites (in bp from an end): Sau3A (20), HaeIII(52), PstI (85), HinfI (181), HaeIII (227). Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 0.35; BamHI--3.6; HindIII--3.6; KpnI--3.6; HincII--3.6. The first half of the dimer is 85% identical to the sequence of the 1 monomer; the second half is 80% identical to the 2 monomer. The insert is a dimer of the 171 bp consensus sequence of alphoid DNA from the subfamily of an alpha-satellite DNA made up of EcoRI dimers or multiples thereof. |
| References： | Carine K, et al. Molecular characterization of human minichromosomes with centromere from chromosome 1 in human-hamster hybrid cells. Somatic Cell Mol. Genet. 15: 445-460, 1989. PubMed: 2781415 Immo E Scheffler, personal communication |