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| 产品名称： | pFa6a-TRP-pADH1-GST |
|---|---|
| 商品货号： | TS194907 |
| Designations： | pFa6a-TRP-pADH1-GST |
| Depositors： | DJ DeMarini |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.0339999198913570 Vector: pFa6a-TRP-pADH1-GST (plasmid) Construction: pCRTOPO2.1 Marker(s):ampR Construct size (kb): 5.033999919891357 Features: marker(s): TRP, ampR tag (N-terminal): GST tag |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--5.0; SalI/BglII--3.4, 1.7; EcoRI--5.0. The marker, promoter and GST cassette is flanked by the F4 primer site (5) and the R4 primer site (3). The size of the F4/R4 PCR product is 2169 nt. F4 primer sequence: 5-(gene-specific sequence)GAATTCGAGCTCGTTTAAAC-3. R4 primer sequence: 5-(gene-specific sequence) ACGCGGAACCAGATCCGATT-3. The gene-specific sequences of these primers are chosen to correspond to the reverse complement of the N-terminal codons of the target gene. Contains a functional truncated (700 nt) ADH promoter. The promoter is not down regulated during the exponential phase. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | DeMarini DJ, et al. Constitutive promoter modules for PCR-based gene modification in Saccharomyces cerevisiae. Yeast 18: 723-728, 2001. PubMed: 11378899 |
| Related Products： | component of:ATCC 87819 |
| Shipped： | freeze-dried |