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| 产品名称： | pMCOA12 |
|---|---|
| 商品货号： | TS194928 |
| Designations： | pMCOA12 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | Codominant segregation of the MspI polymorphism has been observed in 19 3-generation families. TaqI also reveals a polymorphism, but this has not been confirmed by pedigree studies. Otherwise, RFLPs are observed under normal hybridization and wash stringencies. |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 3.0 Gene product: DNA Segment, single copy D9S28 Alleles: A1, A2 |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.7; EcoRI/HindIII--3.0, 2.7; EcoRI/BamHI--5.7; BamHI--5.7; PstI--5.7; AccI--3.7, 1.45, + more; consistent with deposited DNA. Codominant segregation of the MspI polymorphism has been observed in 19 3-generation families. TaqI also reveals a polymorphism, but this has not been confirmed by pedigree studies. The probe should be pre-hybridized with excess human DNA before hybridization. Otherwise, RFLPs are observed under normal hybridization and wash stringencies. Enzyme(s) not detecting polymorphism: BglII, PstI, PvuII, RsaI. |
| References： | Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220 Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence (pMCOA12) on chromosome 9q (D9S28). Nucleic Acids Res. 16: 4744, 1988. PubMed: 2454460 |