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| 产品名称： | pWS4 |
|---|---|
| 商品货号： | TS194929 |
| Designations： | pWS4 |
| GenBank Number： | M24117 |
| Species： | Wound tumor virus |
| Depositors： | DL Nuss |
| Applications： | The internal XbaI site is methylated in HB101 and will not cut if host is dam+. |
| Vector： | Construct size (kb): 5.429999828338623 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.565000057220459 Gene product: segment S4, Pns4 polypeptide |
| Insert Size (kb)： | 2.565 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains internal EcoRI, AvaI, XbaI, SalI, AccI, HincII and PstI sites. The internal XbaI site is methylated in HB101 and will not cut if host is dam+. Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 2.7; EcoRI--5.4, 0.11 (doublet); HindIII--5.6; PstI--3.5, 2.2; XbaI--5.6. Linearize the plasmid with HindIII for in vitro transcription. This is a full-length clone of virus segment S4. The cDNA ends were tailored to terminal BamHI sites before cloning into the BamHI site of pGEM-1. |
| Classification： | Phytoreovirus |
| References： | Xu ZK, et al. Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones. Virology 168: 73-78, 1989. PubMed: 2909991 |