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| 产品名称： | pART1/N795 pART1-N795 |
|---|---|
| 商品货号： | TS195033 |
| Designations： | pART1/N795 pART1-N795 |
| GenBank Number： | M14053 |
| Species： | Rattus norvegicus, rat |
| Depositors： | KR Yamamoto |
| Applications： | in another host, produces protein glucocorticoid receptor |
| Vector： | Construct size (kb): 10.10000038146973 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.799999952316284 Tissue: hepatoma cell line J2.17 (HTC derivative) Gene product: glucocorticoid receptor DNA binding factor 1 GRL Target Gene: glucocorticoid receptor |
| Insert Size (kb)： | 2.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.3, 2.8; EcoRI--4.4, 3.9, 1.2, 0.5; HindIII--4.6, 2.2, 1.7, 0.9, 0.3. The insert contains the following restriction sites (approximate nt from the 5 end): BglI--0.4, 0.6; HindIII--1.0, 1.36, 2.24, 2.4; PstI--1.6, 1.64; EcoRI--2.3. Extends from 24 nt upstream of the major initiation codon to 360 nt downstream of the termination codon and thus encodes a protein of 795 amino acids. pART1 was constructed by inserting a 1.2 kb Schizosaccharomyces pombe ars fragment into the EcoRI site of pUC118 and a 2.2 kb fragment containing Saccharomyces cerevisiae LEU2 into the HindIII site. A 700 bp SphI/PstI fragment containing the adh promoter of Sc. pombe was inserted into polylinker sites. Transcription is driven from the SphI site toward the remaining PstI, SalI and BamHI sites through the insert sequence. |
| References： | Picard D, et al. An inducible expression vector for both fission and budding yeast. Gene 86: 257-261, 1990. PubMed: 2108906 |