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| 产品名称： | Feline leukemia virus |
|---|---|
| 商品货号： | TS195053 |
| Classification： | Retrovirus, MLV-Related Viruses, Mammalian type C subgenera |
| Agent： | Feline leukemia virus |
| Strain： | CT600 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Testicular tissue of asymptomatic Asian leopard cat |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | Vapor phase of liquid nitrogen |
| Comments： | Persistently infected cell culture Cells grow as monolayer Use 0.1% trypsin with 0.02% EDTA to subculture |
| Effect on Host： | No CPE; detect virus by RT assay or antibody |
| Recommended Host： | Cat testicular cells |
| Growth Conditions： | Temperature: 37°C Recommendations For Infection: Virus is persistently infected in a continuous cell line. To passage the virus, trypsinize cell culture with 0.025% Trypsin-EDTA Solution (ATCC® 30-2101™) and seed cells using the growth medium. Incubation: Until cells are confluent |
| Name of Depositor： | S Rasheed, MB Gardner |
| Source： | Testicular tissue of asymptomatic Asian leopard cat |
| References： | Rasheed S, Gardner MB. Isolation of feline leukemia virus from a leopard cat cell line and search for retrovirus in wild felidae. J. Natl. Cancer Inst. 67: 929-933, 1981. PubMed: 6268887 |