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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS195122 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | DG100 CMCC 1961 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried phagemid in E. coli |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | F- thi-1 endA1 supE44 hsdR17 proC::Tn10 lacI+ delta(lacZ)M15 pro- lambda- |
| Type Strain： | no |
| Comments： | It is resistant to tetracycline at 10-20 ug/ml. derived from MM294 and isolated by D. Gelfand A host strain providing efficient transformation and alkaline SDS screening of plasmid recombinants. It has a lac alpha acceptor for blue/white colony discrimination and does not completely shut off lacZ on multicopy plasmids. |
| Medium： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 37.0°C |
| Name of Depositor： | JC Hunter-Cevera |
| References： | Jennie C Hunter-Cevera, personal communication |