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| 产品名称： | CRI-L625 |
|---|---|
| 商品货号： | TS195146 |
| Designations： | CRI-L625 |
| Species： | Homo sapiens, human |
| Depositors： | Collaborative Research, Inc. |
| Vector： | Construct size (kb): 46.0 |
| Insert： | DNA: genomic Insert lengths(kb): 15.0 Tissue: liver Gene product: DNA Segment, single copy D2S38 Alleles: A1, A2, A3, B1, B2, C1, C2 |
| Insert Size (kb)： | 15.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--15.0, 9.0, 7.4, 5.0, 4.0, 2.1, 1.5, 0.66; EcoRI--20.0, 11.0, 7.0 (doublet), 1.4; HindIII--21.0, 7.4, 6.6, 5.8 (doublet); XbaI--21.0, 13.0, 5.2, 2.0, 1.9, 1.2, 0.87, 0.68; KpnI--16.0, 15.0, 4.2, 3.5, 2.5, 1.6, 1.5; SstI--21.0, 9.0, 5.8, 3.8, 1.8 (doublet), 1.6. D2S38 has been mapped to 2q on the human chromosome. The MspI and TaqI polymorphisms have a combined PIC value of 0.57 and a combined heterozygosity of 62%. The TaqI polymorphisms have a combined heterozygosity of 50%. Enzyme(s) not detecting polymorphism: BglII, HincII, EcoRI, RsaI (panel of 5 individuals). |
| References： | Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396 Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638 Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474 |