| 产品名称: | pCMM6 |
|---|---|
| 商品货号: | TS195267 |
| Designations: | pCMM6 |
| Species: | Homo sapiens, human |
| Depositors: | RL White, Y Nakamura |
| Applications: | RFLPs were observed under normal conditions of hybridization and washing. |
| Vector: | Construct size (kb): 6.800000190734863 |
| Insert: | DNA: genomic Insert lengths(kb): 4.0 Gene product: DNA Segment, single copy D20S19 |
| Insert Size (kb): | 4.0 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; EcoRI/HindIII--3.8, 2.95; PstI--3.5, 3.0, 0.67. Codominant segregation demonstrated in 44 3-generation families. Shows tight linkage to the EBN (BFNC) locus, giving a maximal LOD score of 2.87 (theta = 0.039). RFLPs were observed under normal conditions of hybridization and washing. Enzyme(s) not detecting polymorphism: BglII. |
| References: | Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pCMM6) on chromosome 20 D20S19. Nucleic Acids Res. 16: 5222, 1988. PubMed: 2898762 Leppert M, et al. Benign familial neonatal convulsions linked to genetic markers on chromosome 20. Nature 337: 647-648, 1989. PubMed: 2918897 Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 |