宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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rpSag14

货号 TS195349
中文名称 null
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产品名称: rpSag14
商品货号: TS195349
Designations: rpSag14
GenBank Number:

M60737

Species: Rattus norvegicus, rat
Depositors: C Craft
Vector:
Construct size (kb): 4.300000190734863
Insert:
DNA: cDNA
Insert lengths(kb): 1.200000047683716
Tissue: pineal gland
Gene product: S-antigen; retina and pineal gland( S-antigen, retinal S antigen, S-antigen (arrestin); retina and pineal gland, S-antigen, retina and pineal gland) SAG
Insert Size (kb): 1.200
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.3; BamHI--4.3; XbaI--3.7, 0.6; SacI--3.3, 0.6, 0.4.
The insert contains the following restriction sites (approximate kb from the 5 end): SacI--0.4, 0.9; XhoI--.62; PvuI--.82.
The insert extends from nt 163 to 1372 of the reported sequence, containing part of the coding region (nt 28-1239) and a partial 3 untranslated region.
Detects mRNA of 1.7 kb in rat pineal gland.
The 5 end of the insert is closer to the SK primer and the T3 promoter. Transcription from the T3 promoter gives the sense strand.
References:

Craft CM, et al. Differential expression of mRNA and protein encoding retinal and pineal S-antigen during the light/dark cycle. J. Neurochem. 55: 1461-1473, 1990. PubMed: 2213004

Cheryl Craft, personal communication