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微信小章| 产品名称： | Chlamydophila pneumoniae |
|---|---|
| 商品货号： | TS195377 |
| Classification： | Chlamydiaceae, Chlamydophila |
| Deposited As： | Chlamydia pneumoniae Grayston et al. |
| Agent： | Chlamydophila pneumoniae |
| Strain： | TWAR strain TW-183 |
| Applications： | Respiratory research |
| Positive for Mycoplasma： | Yes |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | Note that activities with high potential for aerosol production require BSL 3 facilities and practices. For a time, prior to the acceptance of the C. pneumoniae strains as a separate species, the strains were refered to as TWAR, after the isolates TW-183 and AR-39. AR-39 is available as ATCC 53592. The inclusions are iodine stain negative (contain no glycogen). Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg/mL cycloheximide. Incubate at 37°C for 72 hours. Infection of TC is enhanced by subconfluent monolayer of cells. This preparation has tested positive for Mycoplasma contamination. The inclusions are iodine stain negative (contain no glycogen). The TWAR strains (now C. pneumoniae) of Chlamydia are named after TW183 and AR39. |
| Effect on Host： | Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies |
| Recommended Host： | HEp-2 cells (ATCC® CCL-23™) |
| Growth Conditions： | Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90% - 100% confluent Incubation: 3 days at 35°C, a 5% CO2 in air atmosphere is recommended |
| Name of Depositor： | G Galloway, T Grayston |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Special Collection： | NCRR Contract |
| Source： | Conjunctiva of a child, Taiwan, 1965 |
| Year of Origin： | 1965 |
| References： | Grayston JT, et al. Chlamydia pneumoniae sp. nov. for Chlamydia sp. strain TWAR. Int. J. Syst. Bacteriol. 39: 88-90, 1989. Kuo CC, Grayston JT. Chlamydia spp. strain TWAR: A newly recognized organism associated with atypical pneumonia and other respiratory infections. Clin. Microbiol. Newsletter 10: 137-140, 1988. Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462 type strain |
| Cross References： | Nucleotide (GenBank) : AJ012174 Chlamydophila pneumoniae partial rnpB gene for RNase P RNA subunit. |