微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pHP45omega-Km JF287 |
|---|---|
| 商品货号： | TS195409 |
| Designations： | pHP45omega-Km JF287 |
| Depositors： | J Frey |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.5000000000000000 Vector: pHP45omega-Km (plasmid) Construction: pHP45:omega, Tn5 kanR Marker(s):ampR,kanR Construct size (kb): 4.5 Features: marker(s): ampR, kanR replicon: pMB1 terminator: T4D gene 32 |
| Applications： | integrating vector shuttle vector vector permitting positive selection for integration vector useful for interposon mutagenesis |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.35, 2.35; HindIII--2.4, 2.3; SalI--4.7. Because resistance levels may vary, it is important to determine the proper antibiotic concentration for each strain or isolate. One of a series (TS195409 through 37657) of vectors for in vitro mutagenesis (via the omega interposon) of gram-negative bacteria. pHP45:omega was cut with BssHII + SphI (removing the spcR/strR gene) and the ends were made blunt. This was ligated to a blunt-end 1.5 kb HindIII/SalI fragment of Tn5 containing the kanamycin resistance gene. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Fellay R, et al. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52: 147-154, 1987. PubMed: 3038679 Frey J, Krisch HM. Omega mutagenesis in Gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species. Gene 36: 143-150, 1985. PubMed: 2998930 |
| Shipped： | freeze-dried |