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| 产品名称： | Trimyema shoalsia Nerad and Schaffer |
|---|---|
| 商品货号： | TS195441 |
| Strain Designations： | AP-2 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | organically enriched sediment sample from intertidal zone, south of island cliffs, Appledore Island, ME, 1993 |
| Product Format： | test tube |
| Type Strain： | yes |
| Comments： | species description |
| Medium： | ATCC® Medium 1525: Seawater 802 medium |
| Growth Conditions： | Temperature: 25.0°C Duration: anaerobic Protocol: ATCCNO: 50416 SPEC: Upon receipt aseptically reduce the volume of the culture to 12 ml by removing approximately 2 ml of fluid. Screw the cap on tightly and incubate at 25C on a 5-15 degree horizontal slant. When the bacteria have been largely cleared from the culture (1-2 days), gently invert the culture several times and aseptically transfer 0.1 - 0.3 ml to a fresh tube of ATCC medium 1525 (12 ml of medium in a 16 x 125 mm screw-capped test tube) bacterized with Enterobacter aerogenes ATCC-13048. The medium is bacterized by inoculating a loopful of bacteria into a fresh tube of medium at least 24 hours prior to inoculation with the ciliate. |
| Subcultivation： | Protocol: ATCCNO: 50416 SPEC: Upon receipt aseptically reduce the volume of the culture to 12 ml by removing approximately 2 ml of fluid. Screw the cap on tightly and incubate at 25C on a 5-15 degree horizontal slant. When the bacteria have been largely cleared from the culture (1-2 days), gently invert the culture several times and aseptically transfer 0.1 - 0.3 ml to a fresh tube of ATCC medium 1525 (12 ml of medium in a 16 x 125 mm screw-capped test tube) bacterized with Enterobacter aerogenes ATCC-13048. The medium is bacterized by inoculating a loopful of bacteria into a fresh tube of medium at least 24 hours prior to inoculation with the ciliate. |
| Name of Depositor： | EB Small |
| Chain of Custody： | ATCC < |
| Year of Origin： | 1993 |
| References： | Nerad TA, et al. Trimeya shoalsia sp. n., an anaerobic microaerotolerant marine cillitate from Aplledore Island, Gulf of Maine, USA. Acta Protozool. 34: 289-298, 1995. type strain |