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| 产品名称： | pCMV-Cip1 |
|---|---|
| 商品货号： | TS195476 |
| Designations： | pCMV-Cip1 |
| GenBank Number： | L25610 |
| Species： | Homo sapiens, human |
| Depositors： | SJ Elledge |
| Applications： | in another host, produces protein cyclin-dependent kinase inhibitor 1A (p21, Cip1) melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein) |
| Vector： | Construct size (kb): 5.139999866485596 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSXV Intact vector size: 4.200 Type of vector: plasmid Vector end: XhoI Vector end: BamHI Cloning sites: XhoI Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: CMV replicon: pMB1 terminator: tat Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CDKN1A Other gene symbols: CIP1; WAF1* Gene name: cyclin-dependent kinase inhibitor 1A (p21, Cip1) Contains complete coding sequence?: Y Chromosome: 6; Localization: 6 p21.2 Tissue: B-lymphocyte (EBV-transformed) Type of DNA: cDNA Insert 5 end: XhoI Insert 3 end: BglII Insert size (kb): 1.64 Cross references: DNA Seq. Acc.: L25610 Insert lengths(kb): 1.639999985694885 Tissue: B lymphocyte (EBV transformed) Gene product: cyclin-dependent kinase inhibitor 1A (p21, Cip1)( melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein)) CIP1 Target Gene: cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
| Insert Size (kb)： | 1.640 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--1.6, 1.1, 0.6, 0.5 (doublet), 0.3 (doublet), 0.2; EcoRI--4.4, 0.9; KpnI--4.8, 0.25; PvuII--4.0, 1.2; XhoI--5.2; XbaI/NdeI--2.7, 2.0, 0.45; EcoRI/XhoI--3.3, 1.05, 0.9. Digestion with EcoRI+XhoI yields a 1 kb fragment that can be used as a probe. Contains the complete coding sequence (nt 79-573 of the sequence record). When transfected into human cells, expresses the protein from a CMV promoter. |
| References： | Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751 Stephen J Elledge, personal communication |