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| 产品名称： | Influenza A virus (H1N1) |
|---|---|
| 商品货号： | TS195624 |
| Classification： | Orthomyxoviridae, Influenzavirus A |
| Deposited As： | Influenza A virus |
| Agent： | Influenza A virus (H1N1) |
| Strain： | A/WS/33 |
| Applications： | Influenza Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation of egg allantoic fluid product ATCC® VR-825™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-825™ and ATCC® VR-1520™ have not been compared for sequence or infectivity in eggs and tissue culture. Demonstrates cross-reactivity with Influenza A virus, A/PR/8/34 and swine influenza. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： | MDCK (ATCC® CCL-34™) |
| Growth Conditions： | Temperature: 33°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days
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| Name of Depositor： | WA Chappell, Centers for Disease Control and Prevention; ATCC |
| Special Collection： | NCRR Contract |
| Source： | Throat washings from an influenza patient |
| References： | Burnet FM. A genetic approach to variation in influenza viruses; the characters of three substrains of influenza virus A (WS). J. Gen. Microbiol. 5: 46-53, 1951. PubMed: 14824469 Smith W, et al. A virus isolated from influenza patients. Lancet 2: 66-68, 1933. |