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| 产品名称： | RK2 |
|---|---|
| 商品货号： | TS195641 |
| Designations： | RK2 |
| Depositors： | DR Helinski |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 56.0000000000000000 Vector: RK2 (plasmid) Construction: natural isolate Marker(s):ampR,kanR,tetR Construct size (kb): 56.0 Features: marker(s): ampR, kanR, tetR replicon: RK2 |
| Applications： | shuttle vector |
| Comments： | natural isolate from Pseudomonas sp. contains relaxation complex site (rlx). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Meyer R, et al. Molecular vehicle properties of the broad host range plasmid RK2. Science 190: 1226-1228, 1975. PubMed: 1060178 Ditta G, et al. Broad host range DNA cloning system for Gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA 77: 7347-7351, 1980. PubMed: 7012838 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |