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微信小章| 产品名称： | TH-1, Subline B1 |
|---|---|
| 商品货号： | TS195668 |
| Organism： | Terrapene carolina, turtle, box |
| Tissue： | heart |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Virus Resistance： | poliovirus 1 |
| Comments： | Growth is inhibited at alkaline pH. Medium should be adjusted to neutrality after plating. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Every 10 to 14 days Remove medium, add fresh 0.25% trypsin for 2 to 3 minutes, remove trypsin and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks. |
| Name of Depositor： | HF Clark |
| Deposited As： | Terrapene carolina |
| References： | Clark HF, Karzon DT. Acquired tolerance to elevated temperatures in a poikilothermic cell line (terrapene heart, TH-1). Exp. Cell Res. 48: 269-275, 1967. PubMed: 6082311 Clark HF, Karzon DT. Terrapene heart (TH-1), a continuous cell line from the heart of the box turtle Terrapene carolina. Exp. Cell Res. 48: 263-268, 1967. PubMed: 4295486 Huang CC, Clark HF. Chromosome changes in cell lines of the box turtle (Terrapene carolina) grown at two different temperatures. Can. J. Genet. Cytol. 9: 449-461, 1967. PubMed: 6082587 |