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| 产品名称： | RR1 |
|---|---|
| 商品货号： | TS195704 |
| Designations： | RR1 |
| GenBank Number： | S70522 |
| Depositors： | DH Jones |
| Applications： | vector useful for site-directed mutagenesis |
| Vector： | Construct size (kb): 1.914000034332275 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--1.9; XhoI--1.9; BamHI--1.9. Insert contains the following restriction sites (approximate kb from nt 1): AvaI--0.05, 0.09; BamHI--0.05; BglII--0.04; BssHII--0.01; DraI--0.92, 0.94, 1.63; KpnI--0.11; NotI--0.17; SmaI--0.09; SspI--1.86; XbaI--0.10; XhoI--0.05. Cloning vector containing a reduced number of restriction sites, useful for site-directed mutagenesis of inserts. Constructed from pUC19 by elimination of many of the restriction endonuclease sites and replacement of nt 2555-640 with a smaller polylinker. Differs from RR2 (ATCC 87077) by a T1100C transition and the loss of an FspI restriction site at nt 1280. |
| References： | Jones DH, et al. Production of a vector to facilitate DNA mutagenesis and recombination. BioTechniques 16: 694-701, 1994. PubMed: 8024792 |