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| 产品名称： | pExSec1 |
|---|---|
| 商品货号： | TS195726 |
| Designations： | pExSec1 |
| Depositors： | C Bruenen-Nieweler |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 3.3810000419616700 DESCRIPTION OF VECTOR: Intact vector size: 3.381 Type of vector: phagemid Cloning sites: EcoRI SacI Asp718 KpnI SmaI BamHI Polylinker sites: EcoRI SacI Asp718 KpnI SmaI BamHI Construction: pUK21, pET3b, pEZZ18 Host range: Escherichia coli Features (with orientation and position when available): promoter for in vitro transcription: T7, ->, 82-98 ribosome-binding site: Shine-Dalgarno, ->, 148-153 restriction site: NdeI, 159-164 leader peptide: synthetic ZZ sequence of protein A, ->, 162-641 MCS: EcoRI...BamHI, ->, 642-670 transcription terminator: T7, ->, 673-795 other: M13 intergenic region, -> marker(s): kanR, -> replicon: M13 Vector: pExSec1 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pUK21, pET3b, pEZZ18 Marker(s):kanR Construct size (kb): 3.38100004196167 Features: leader peptide: synthetic ZZ sequence of protein A marker(s): kanR other: M13 intergenic region promoter for in vitro transcription: T7 replicon: M13 MCS: EcoRI...BamHI restriction site: NdeI ribosome-binding site: Shine-Dalgarno transcription terminator: T7 |
| Applications： | expression vector vector permitting RNA synthesis in vitro vector permitting construction of fusion proteins vector permitting production of single-stranded DNA vector with sequences for protein export |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.4; BglI/EcoRI--3.1, 0.3; EcoRI--3.4; BglII/EcoRV--3.2, 0.2. Expression and secretion vector for E. coli. Constructed using M13 replication and kanR selection mechanisms from pUK21, T7 regulatory sequences from a 271 bp BglII-EcoRV fragment of pET3b and leader peptide sequence and multiple cloning sites from pEZZ18. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Brunen-Nieweler C, et al. Expression of the pheromone 3-encoding gene of Euplotes octocarinatus using a novel bacterial secretion vector. Gene 150: 187-192, 1994. PubMed: 7959050 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |