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| 产品名称： | Xenopus laevis NotI/SalI |
|---|---|
| 商品货号： | TS195759 |
| Designations： | Xenopus laevis NotI/SalI |
| Species： | Xenopus laevis, frog, South African clawed |
| Depositors： | MJ Castanon |
| Origin： | oocyte |
| Vector： | Tissue: oocyte DNA: cDNA Insert size range: 1 - 2 kb Genome Equivalent: 0.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | xa0Frozen glycerol stock of library in Escherichia coli HB101. |
| Comments： | Constructed using a SalI-containing oligo(dT) primer and adaptors to result in 5NotI and 3SalI ends. pMC944 is a YE-type vector permitting expression from the mating factor alpha 1 promoter. Unique XbaI and SphI sites in the promoter and ADH2 terminator, respectively, allow transfer of the expression cassette. The vector does not contain an initiation codon. It must be provided by the cDNA. Verified by PCR to contain clones for B4, D7, enolase, Eg1, and nucleoplasmin. The low abundance Vg1 was not detected. |
| References： | Spevak W, et al. Saccharomyces cerevisiae cdc15 mutants arrested at a late stage in anaphase are rescued by Xenopus cDNAs encoding N-ras or a protein with beta-transducin repeats. Mol. Cell. Biol. 13: 4953-4966, 1993. PubMed: 8393141 |