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| 产品名称： | Hybrid Moloney/Amphotropic murine leukemia virus (Mo/A-MuLv) |
|---|---|
| 商品货号： | TS195762 |
| Classification： | Retrovirus, Mammalian Type C Retrovirus, Mammalian Group, Amphotropic |
| Agent： | Hybrid Moloney/Amphotropic murine leukemia virus (Mo/A-MuLv) |
| Strain： | 4070A envelope strain |
| Applications： | Analytical methodologies Pharmaceutical and Personal Care |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Vapor phase of liquid nitrogen |
| Comments： | This virus produces foci of round cells on MiCL1(Mink S+L-) cell line, and plaques on PG-4 (feline S+L- cell line). This holding consists of persistently infected tk(-)NIH3T3 producer cells that shed the Mo/A-MLV virus.
Amphotropic |
| Effect on Host： | No CPE in NIH3T3 cells |
| Recommended Host： | Recommended Host: This virus is continuously produced by the cell line in which it is shipped. No additional host is required; however, if desired the virus may be used to infect other cells, which are listed as alternate hosts. Alternate Host(s): Many mammalian cell lines (murine, rat, human except certain bovine and hamster cells) |
| Growth Conditions： | Incubation: 2 to 7 days at 37°C, a 5% CO2 in air atmosphere is recommended |
| Name of Depositor： | CA Wilson |
| Source： | Transfection of tk(-)NIH3T3 cells with molecular clone of MLV derived by A. Dusty Miller |
| References： | Miller AD, et al. Generation of helper-free amphotropic retroviurses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene. Mol. Cell. Biol. 5: 431-437, 1985. PubMed: 2985952 General Chapters: <1046> Cell and Gene Therapy Products - Analytical Methodologies. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011 |