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| 产品名称： | pJB8 |
|---|---|
| 商品货号： | TS195790 |
| Designations： | pJB8 |
| Depositors： | D Ish-Horowicz, JF Burke |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.4000000953674320 Vector: pJB8 (cosmid) Construction: HomerI Marker(s):ampR Construct size (kb): 5.400000095367432 Features: marker(s): ampR replicon: pMB1 |
| Applications： | vector for constructing genomic libraries |
| Comments： | Restriction digests analyzed on agarose gels give the following sizes (kb): HindIII--5.4; BamHI--5.4; PstI/HindIII--4.6, 0.8; PstI/SalI--3.3, 2.3. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. A cosmid vector containing a single cos site. Cloning into the BamHI or EcoRI site involves 2 preparations of dephosphorylated vector, one linearized with HindIII, the other linearized with SalI. An equimolar ratio of right-hand and left-hand cos containing vector fragments, digested with BamHI or EcoRI can be ligated to appropriately digested and dephosphorylated inserts. Only molecules with 2 cos sites separated by 37-50 kb can be packaged. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Ish-Horowicz D, Burke JF. Rapid and efficient cosmid cloning. Nucleic Acids Res. 9: 2989-2998, 1981. PubMed: 6269067 |
| Shipped： | freeze-dried |