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| 产品名称： | pCMV HSV S2P plasmid in Escherichia coli |
|---|---|
| 商品货号： | TS195862 |
| Designations： | pCMV HSV S2P plasmid in Escherichia coli |
| GenBank Number： | AF019612 |
| Species： | Homo sapiens, human |
| Depositors： | JL Goldstein |
| Applications： | encodes an epitope tag for protein isolation or monitoring S2P |
| Vector： | Vector: pcDNA3 Vector type: phagemid Vector size (kb): 5.4 Markers: ampR Features: CMV promoter, T7 and SP6 promoters, SV40 replicon, G418 resistance, pMB1 replicon |
| Insert： | Insert size (kb): 1.7 Gene: S2P DNA: cDNA Source : human Genbank accession: AF019612 Insert end 5’: NheI Insert end 3’: XbaI |
| Insert Size (kb)： | 1.7 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Freeze dried E. coli containing the plasmid |
| Comments： | Restriction digests of the clone give the following sizes (kb): NheI/XbaI--5.4, 1.7; PstI--4.0, 3.1; ClaI--7.1; EcoRI--7.1. The insert contains the following restriction sites (approximate kb from the 5 end): StyI--0.36; DraII--0.90; BglI--1.40; SacI--1.46. The insert contains human S2P with HSV epitope tag at the amino terminus. |
| References： | Rawson RB, et al. Complementation cloning of S2P, a gene encoding a putative metalloprotease required for intramembrane cleavage of SREBPs. Mol. Cell 1: 47-57, 1997. PubMed: 9659902 |