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| 产品名称： | Streptococcus pneumoniae (Klein) Chester |
|---|---|
| 商品货号： | TS195895 |
| Strain Designations： | pLS101 825 |
| Application： | Shuttle vector Respiratory research |
| Isolation： | Derived from existing strain |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | 2°C to 8°C |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Preceptrol®： | no |
| Type Strain： | no |
| Comments： | Pls101 contains the following restriction sites (kb markers according to the published map): PstI--0/6.4, 0.8; BclI--1.8, 2.8; HpaII--2.4; EcoRI--3.0, 4.1; HindIII--4.2, 4.9, 5.1, 5.3, 5.5, 6.2; KpnI--4.75; SacI--5.6. Cloning into the unique HpaII site inactivates tet. Removal of the smaller BclI fragment also inactivates tet. Ecori digestion removes a dispensible EcoRI fragment. Cloning into the unique KpnI and SacI sites inactivates malM. Cloning into the 4.2 kb vector fragment resulting from HindIII digestion removes malM. Mal- recombinants can be enriched by penicillin selection of tetR bacteria. Strain is noncapsulated and reportedly never reverts to encapsulated form. Strain is noncapsulated and reportedly never reverts to encapsulated form This was constructed from pLS69 (ATCC 39937) by ligating a partial 2.0 kb HindIII fragment containing malM to a 4.4 kb HindIII from a complete digest of pLS69. The latter fragment contains tet as well as the origin of replication. |
| Medium： | ATCC® Medium 1420: Trypticase soy agar with defibrinated sheep blood, sucrose and 0.5 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Aerobic |
| Name of Depositor： | SA Lacks, SA Lacks |
| U.S. Patent Number： | |
| References： | Balganesh TS, Lacks SA. Plasmid vector for cloning in Streptococcus pneumoniae and strategies for enrichment for recombinant plasmids. Gene 29: 221-230, 1984. PubMed: 6092224 derived from Streptococcus pneumoniae R6 |