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| 产品名称： | Chlamydia trachomatis |
|---|---|
| 商品货号： | TS195959 |
| Classification： | Chlamydiaceae, Chlamydia |
| Deposited As： | trachoma serotype k |
| Agent： | Chlamydia trachomatis |
| Strain： | Trachoma type K strain UW-31/Cx |
| Applications： | Note that activities with high potential for aerosol production require BSL 3 facilities and practices. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Closely related immunologically with LGV Type L III. Note that activities with high potential for aerosol production require BSL 3 facilities and practices. Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 ug/mL cycloheximide. Incubate at 37°C for 48 hours. Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host： | Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa |
| Recommended Host： | McCoy cells (ATCC® CRL-1696™)
HeLa 229 cells (ATCC® CCL-2.1™) |
| Growth Conditions： | Temperature: 36.5°C Incubation: 2 days at 37°C |
| Name of Depositor： | SP Wang |
| Source： | Human cervix, cervicitis, Seattle, WA |
| References： | Kuo CC, et al. TRIC type K, a new immunologic type of Chlamydia trachomatis. J. Immunol. 113: 591-596, 1974. PubMed: 4210884 |