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| 产品名称： | pHIIF-SV-gamma1 HIIF-A |
|---|---|
| 商品货号： | TS195980 |
| Designations： | pHIIF-SV-gamma1 HIIF-A |
| Species： | Homo sapiens, human |
| Depositors： | Biogen N.V., Biogen, Inc., Biogen N.V. |
| Applications： | produces protein interferon gamma |
| Vector： | Construct size (kb): 9.699999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pSV529 Intact vector size: 8.640 Type of vector: plasmid Vector end: BamHI (C-tailed) Vector end: BamHI (C-tailed) Cloning sites: BamHI EcoRI Polylinker sites: Construction: pBR322, SV40, pSV52 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: SV40 late replicon: pMB1, SV40 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: IFNG Genomic copy number: unique Gene name: interferon, gamma Contains complete coding sequence?: Y Chromosome: 12; Localization: 12 q24.1 Tissue: splenocytes, mitogen-stimulated Type of DNA: cDNA Insert end: Modification: GC tails Insert end: Modification: GC tails Insert size (kb): 1.1 Cross references: Insert lengths(kb): 1.100000023841858 Tissue: splenocytes, mitogen-stimulated Gene product: interferon gamma IFNG Target Gene: interferon gamma |
| Insert Size (kb)： | 1.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--9.2, 5.5, 2.7, 1.1; BglI--3.3, 2.5, 1.35; NcoI--4.4, 3.1, 1.9. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.86; NcoI--0.9. Analysis of the undigested plasmid shows a significant band migrating at approximately 2.7 kb and a weaker band at 5.5 kb. Contains the complete coding sequence, between nucleotides (approx) 100 and 600, with expression regulated by the late SV40 promoter in transfected cells. |
| References： | Fiers WC, Allet B. DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields. US Patent 5,004,689 dated Apr 2 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |